December 06, 1997

Article at New Scientist

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FEATURE | On the trail of the lonesome pine

David Nobel with some of the trees grown from cuttings and seeds from the original stand of Wollemia nobilis: a new genus of pine uncovered on the doorstep of Sydney

They should have died out with T. rex, but their seedlings are alive and well in nurseries around the world.

By Wilson da Silva

IT HAS been called the botanical find of the century: a lonely stand of conifers, the last of their kind and thought to have been extinct for aeons-until three bushwalkers came across them one sunny winter afternoon.

The 23 odd-looking pines, stretching up to 40 metres through the canopy of a deep and inaccessible gorge in Australia’s Wollemi National Park, are from a group that once covered the southern supercontinent of Gondwana. The trees were thought to have disappeared long before humans walked the Earth-until David Noble and his companions stumbled upon them. “They just looked a bit out of the ordinary,” says Nobel. “So I popped a cutting in my pack and took it back home to try and identify it.”

Back at his Blue Mountains home west of Sydney, the keen canyoner and student of botany was unable to match the sample with anything in the textbooks, so he later showed it to Wyn Jones, a colleague at the National Parks and Wildlife Service. Jones has spent most of his life studying rare plants of the Blue Mountains. “I thought that it was just a fern,” he says recalling that morning in August 1994. “Until Nobel said, `It’s a bloody great tree!’. And that sort of shocked me.”

To reach the site, the search party had to abseil 400 metres down the side of a precipitous gorge in stages.

A month later the two retraced Nobel’s original route back to the site to collect more specimens. This was no easy task: the Wollemi National Park is a sandstone massif covered in eucalyptus woodland and scrub, peppered with isolated patches of rainforest. Its 496 000 hectares are criss-crossed with hundreds of densely vegetated canyons ranging from a few metres to many hundreds deep. To reach the site, the search party had to abseil 400 metres down the side of a precipitous gorge in stages, and then pick their way through thick foliage alongside a creek.

Once there, Jones realised that he had surveyed this area from the air some five years before, coming within a few hundred metres of the pines but not noticing them. Up close, however, they were distinctly unusual. Strange nodules covered the trunks, making the bark look like bubbling chocolate.

Wyn Jones and David Noble of the NSW Parks and Wildlife Service

What that research reveals are that the trees, named Wollemia nobilis -partly in homage to Noble and partly because of their majesty-are both surprisingly unusual and remarkably ordinary. The Wollemi pine is a conifer, a group of seed-bearing trees dating back to the Carboniferous era more than 300 million years ago. It is a member of the ancient conifer family of Araucariaceae, until now thought to include only two surviving genera: Araucaria and Agathis. In many ways, the new genus appears to be somewhere between these two.

“This reminds us that we don’t know as much about the world as we keep thinking we do.”

The Araucariaceae family first appears in the fossil record in the Triassic, 250 million years ago-around the time that the sandstone massif of the Wollemi National Park emerged. It seems to have been most widespread between 200 and 65 million years ago, from the Jurassic to the Cretaceous periods, when it grew all over the planet. Then, at the end of the Cretaceous, just when the last big wave of extinctions claimed the dinosaurs, it began a dramatic retreat and disappeared from the northern hemisphere altogether.

Missing records

Nonetheless, the family remained an important part of the forests of the vast southern supercontinent of Gondwana until the middle of the Tertiary, some 30 million years ago. Then began a slow decline in range and diversity, as flowering plants came into their own.

The fossil record contains no Wollemi pines. The closest match yet found is between Wollemi pollen and fossilised pollen that was previously something of a mystery. Known as Dilwynites, it had been considered part of the ancient conifer family of Araucariaceae, but no one could agree on which genus.

A Wollemi seed cone

From then on, the fossil record is silent. Everyone had assumed that the genus to which Wollemi pines belong had been extinct for millions of years-just one more curiosity for botanists to argue over. So how did this single stand of trees survive? And why? Here the mystery deepens.

Secret gorge

Since the pine’s discovery, botanists have scoured the park for more of the ancient trees. In three years they have uncovered just one more stand of 17 trees, at an even more secluded spot about a kilometre upstream from the first-the exact whereabouts of the gorge is kept secret to protect the trees. If they were once abundant throughout the continent, why have they survived the millennia in this one rainforest nook among the many rainforests in the southeast and northeast of Australia? And why in this one isolated gorge?

“It’s just there, and we have no real understanding of why,” says plant pathologist Brett Summerell of the Royal Botanic Gardens. “It’s in a very deep gorge, but there are others next door that are just as deep and just as inaccessible; there are similar soils, similar light regimes and similar creeks running in the same direction. Apart from the Wollemi pines being there, it’s like any other gully in the Wollemi National Park or the [nearby] Blue Mountains National Park. It’s very puzzling.” Summerell suggests that unlike its closest relatives in the same genus, the Wollemi pine may never have been very widespread. If it was highly specialised to fit a particular ecological niche, it could have been confined to the wet eastern rainforests of Australia. Such a limited distribution would have made eventual isolation more likely.

From then on, the fossil record is silent. So how did this single stand of trees survive? And why? Here the mystery deepens.

Whatever the history of the genus, there is no doubt that the remaining trees have been isolated for a very long time. To find out exactly how long, ecological geneticist Rod Peakall and his colleagues at Canberra’s Australian National University have spent the past year studying the genetic diversity of the genus. Because inbreeding reduces genetic diversity over time, establishing how much variation there is between members of a particular group allows scientists to estimate how long a species has been reproducing within that narrow population.

So Peakall’s team analysed genetic markers in eight adult trees from the first stand and four from the second. Using a standard assay, allozyme electrophoresis, the team searched the DNA for differences in allozymes, the genes that code for enzymes. They compared between 30 and 40 sites on the genomes of each of the 12 samples. The result: zero variation. This was surprising, but not astounding: small, isolated populations tend to have limited genetic diversity, and their assay has on rare occasions failed to find any variation. For a more detailed look, the team used a recent version of DNA fingerprinting called AFLP (amplified fragment length polymorphism), which compares thousands of points on a genome. This technique, which is 50 times more powerful than the allozyme method, has always turned up trumps.

The original stand of Woollemi Pines, found in an isolated canyon

Complete clone

But not this time. Peakall was left stumped. With no differences in the genes, and without baseline data on normal levels of variability, it was impossible to establish how long the trees had been isolated. “How can you put a comparison on zero?” says Peakall.

While the search for genetic variability is not over yet, the results strongly suggest that the pines have been a small population isolated for millennia. The other possibility is that trees are in reality clones of a single remnant organism that has been propagating purely through coppicing for a very long time. Here, the tree extends its roots horizontally and grows another stem, which then develops into a fully fledged tree that loses the original root linkages over time.

At one of the sites, a group of 160 stems appear to be part of a single individual. Such trees may be extremely old. “There’s no doubt that with the Wollemi pine, there’s local cloning caused by this root coppicing,” says Peakall. “The question is whether it goes beyond that: is there an underground root system, and were they once all the same organism?” But proving this latter theory might require uprooting some of the trees, which are considered too precious.

If they are indeed clones, the fact there are two sites some distance apart makes it hard to imagine that all the trees came from one individual. The lack of genetic diversity may be a combination of both isolation and clonality. And the cloning process itself gives a clue about how old the stands may be: non-sexual propagation on this level would take many generations to develop.

Assuming similar growth rates to that in related genera, the oldest trees are at least 1000 years old. Peakall’s best guess is that they have probably been isolated for between 6000 and 10 000 years. “Whether this stand has been isolated for two million years or less we just cannot tell,” says Blaxell. “I’d say it’s probably tens of thousands, if not hundreds of thousands of years.”

Seeds of success

What has confused researchers is the ease with which they have been able to cultivate new pines from seed. They had feared that the decline of Wollemi pines, and their apparent enthusiasm for asexual reproduction, might be the result of poor seed viability. But over the past two years, more than 1300 seedlings have grown successfully at various greenhouse sites. Many of the saplings are more than a metre tall and show no difficulty coping with conditions outside the Wollemi National Park.

If sexual reproduction functions adequately, why does the Wollemi pine endlessly clone itself? “This natural clonal capacity is interesting,” says Peakall. “You can actually force some of the other conifers to do the same thing by chopping them down or causing other major disturbance. But they won’t do it naturally, which this seems to do. It’s meant that it has been able to hang in there for a very long time and not be reliant on sexual reproduction.”

Plant pathologist Brett Summerell

The clonal nature of the trees, if proven, makes it even more imperative that they are protected because any introduced disease that killed one would probably kill them all. The State of New South Wales has special laws protecting the pines, as well as a big recovery plan. “There are a number of programmes under way looking at what conditions they grow best under, what sort of pH in the soil, what sort of fertilisers and light conditions,” says Summerell. “It grows quite well from both side-shoot cuttings and top-growth cuttings, and grows quite well from seeds, which can be stored using relatively conventional techniques.”

When the recovery programme began, little was known about the Wollemi. Botanists feared that climbing the trunk with spiked boots to collect cones from near the tree’s apex might be damaging. So seeds were collected from treetops by volunteers swinging from helicopters. This proved dangerous and not very effective: when the helicopters came close, the downwash blew away many cones, and when they moved higher, the volunteers on wires would sway precariously. Finally, the botanists put nets beneath the trees to collect the seeds.

“Part of the conservation and preservation strategy is to get it out as widely as possible, so anyone can buy one,” says Blaxell. This is not just a money-making venture; it’s also the best guarantee that the original site will remain protected. “The Wollemi has generated so much interest that unscrupulous collectors might go to the site and damage the trees or introduce some disease. We don’t want to lose it,” Blaxell says.

For now, anyone curious to see this biological time capsule from the Jurassic can go to the Royal Botanic Gardens in Sydney, where two juvenile pines are growing behind glass. In September, London’s Kew Gardens received a pair of saplings that will eventually go on display, and gardens around the world will be sent specimens in the next year. The Wollemis, only a few years ago staring at the abyss of extinction, now have a second chance.